Tracing magnetically labeled mesenchymal stem cells transplanted into rat livers by MRI / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 170-173, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-285437
ABSTRACT
<p><b>OBJECTIVE</b>To trace magnetically labeled MSCs transplanted into the rat livers by magnetic resonance imaging (MRI).</p><p><b>METHODS</b>Feridex and DAPI labeled rat mesenchymal stem cells (MSCs) were injected via portal veins into carbon tetrachloride treated rats. MRI was performed with a clinical 1.5 T MRI machine immediately before the MSCs injection and at h 1, d 3, d 7, and d 14 after the injection, and then the signal-to-noise ratio (SNR) was measured. MRI findings were compared with the liver histopathologies after the slides were stained with fluorescence dye and Prussian blue.</p><p><b>RESULTS</b>The SNR for liver was 1.10+/-0.26 at hour 1, 8.18+/-1.55 at day 3, 11.08+/-1.30 at day 7, and 14.15+/-1.02 at day 14 respectively. Within 7 days after the MSCs transplantation, the SNRs of the livers were significantly lower than those before the transplantation (P less than 0.05). Histologically, the blue fluorescent particles under the fluorescence microscopy matched in distribution with the iron particles on the Prussian blue stained slides.</p><p><b>CONCLUSION</b>The magnetically labeled MSCs transplanted into livers give rise to an obvious signal decrease, and can be tracked with a 1.5 T clinical MRI machine for up to 7 days after MSCs transplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Radioactive Tracers
/
Magnetic Resonance Imaging
/
Image Enhancement
/
Rats, Wistar
/
Mesenchymal Stem Cell Transplantation
/
Liver
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2007
Type:
Article
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