Identification of protein peroxiredoxin 2 related to crystalline NiS-induced neoplastic transformation / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 267-270, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-285908
ABSTRACT
<p><b>OBJECTIVE</b>To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.</p><p><b>METHODS</b>Two dimensional electrophoresis (2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.</p><p><b>RESULTS</b>The good 2-DE pattern including resolution and reproducibility was obtained. Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights (MW) ranging from 14,400 to 94,000 KD and pI 3 - 10. A protein PDX2 with MW 21,890 KD, pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.</p><p><b>CONCLUSION</b>PDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bronchi
/
Cell Line
/
Cell Transformation, Neoplastic
/
Proteome
/
Cell Biology
/
Epithelial Cells
/
Toxicity
/
Peroxiredoxins
/
Metabolism
/
Nickel
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2005
Type:
Article
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