cDNA microarray to identify the significance of DNA replication and damage repair genes associated with benzene poisoning / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screen DNA replication, and damage repair genes associated with benzene poisoning by using gene expression profile analysis.</p><p><b>METHODS</b>Leucocytes in peripheral blood of seven patients and seven normal controls were collected and total RNA was extracted. The cDNA probes were prepared by labeling cell RNA with Cy3-dUTP and Cy5-dUTP with reverse transcription. The arrays with 141 genes were hybridized against the cDNA probe mixture, and the fluorescent signals were scanned.</p><p><b>RESULTS</b>Twenty five differentially expressed genes were screened out. Among these genes, 16 genes were up-regulated and 9 down-regulated. They were DNA replication genes such as PRIM2A, ORC1L etc; DNA synthesis, recombination and repair genes such as POLK etc; DNA damage signaling/repair proteins and DNA ligases such as RECQL, PRKDC, G22P1, ERCC3, ERCC1, CRY1, CHES1, BRCA2, APEX etc; proteins involved in recombination such as RECQL; and other DNA synthesis, recombination, and repair proteins such as SKIV2L, RBMS1, SON, SET.</p><p><b>CONCLUSIONS</b>Some DNA replication, and damage repair genes associated with benzene poisoning show differential expression, which provides the basis for screening biomarkers of benzene poisoning.</p>