Role of Dlx1 natural antisense transcript in mice brain development / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the role of Dlx1as, a natural antisense transcript, in mice brain development.</p><p><b>METHODS</b>Based on the bioinformatic and molecular biological findings, we confirmed whether the Dlx1as has a poly-A tail. The temporal expressions of Dlx1as and Dlx1 mRNA were determined by quantitative real-time PCR and the spatial expressions of Dlx1as and Dlx1 mRNA were compared by using in situ hybridization.</p><p><b>RESULTS</b>Dlx1as had a poly-A signal. Dlx1as's expression was at a high level through a period of time in the embryo development, which was similar with Dlx1 mRNA in temporal but different in spatial.</p><p><b>CONCLUSIONS</b>Dlx1as is highly expressed in a certain period during mice brain development, which spatially supplements the mRNA expression of D1x1. Therefore, it is supposed that Dlx1as influences the mice brain development by regulating Dlx1 mRNA.</p>