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A novel method for multiplex detection of gastroenteritis-associated viruses / 病毒学报
Chinese Journal of Virology ; (6): 288-293, 2011.
Article in Chinese | WPRIM | ID: wpr-286041
ABSTRACT
To develop and optimize a simultaneous detection method of RotavirusA, Norovirus GI, GII, Sapovirus, human astrovirus, enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system. The sensitivity was verified to be 10(4) copies/microL with plasmids containing the viral targets in triplicate on different days, and no cross-reaction with enterovirus71, human Parechovirus and PicobirnavirusII was observed. Finally, we successfully developed a high throughout, rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis, which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Viruses / Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction / Gastroenteritis / Methods Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Virology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Viruses / Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction / Gastroenteritis / Methods Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Virology Year: 2011 Type: Article