Isolation and characterization of an iridovirus from sick giant salamander (Andrias davidianus) / 病毒学报
Chinese Journal of Virology
;
(6): 274-282, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-286043
ABSTRACT
A virus was isolated from cultured sick giant salmander (Andrias davidianus ) in a farm, Shanxi Province, China. Skin ulceration and necrosis of the distal limbs are main clinical symptoms. Virus propagated and caused CPE at 10 degrees C to 30 degrees C in BF-2, CO, CHSE, FHM cells. The optimum condition of replication was in BF-2 cells at 25 degrees C. The virus was proved to be senstive to chloroform, heat, pH3 and pH10 treatment. Viral replication was inhibited by 5-Fluoro-2-deoxyuridine (FUDR). These results indicated that the virus possessed an envelope and DNA as the genome. Electron-microscopic observation of thin-section showed numerous hexagonal viral particles measuring 130 nm to 150 nm in diameter orderly arranged in a lattice form in cytoplasm of BF-2 cells. The particles showed typical iridovirus morphology. A 413 bp fragment was amplified from the viral main capsid protein gene by PCR. The fragments was sequenced and analysed. The results showed the isolate shared more than 96% nucleotide identity with some Ranaviruses. We suggested that this virus was named as Andrias davidianus iridovirus (ADIV) tentatively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urodela
/
Virology
/
Molecular Sequence Data
/
Base Sequence
/
Iridovirus
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2011
Type:
Article
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