Detection of molecular markers of amantadine resistance in avian influenza viruses by pyrosequencing / 病毒学报
Chinese Journal of Virology
;
(6): 392-395, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-286106
ABSTRACT
Mutation in any of five key amino acid residues (at positions 26, 27, 30, 31 and 34) within the M2 protein of influenza A viruses leads to resistance against the amantodine class of anti-influenza drugs. In this study, a pyrosequencing method was described to rapidly detect established five molecular markers of resistance to M2 blockers, amantadine. The residues L26, V27, A30, S31 and G34 in the M2 protein were targeted for pyrosequencing, and 94 avian influenza viruses were used to perform the amantadine resistance analysis. Our results showed that most of avian influenza viruses were amantadine resistant, Mutations V27I and S31N were founded in these isolates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Influenza A virus
/
Virology
/
Amantadine
/
Chickens
/
Reverse Transcriptase Polymerase Chain Reaction
/
Drug Resistance, Viral
/
Therapeutic Uses
/
Drug Therapy
/
Influenza in Birds
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2010
Type:
Article
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