Expression of CD38 and HLA-DR on CD8+ T cells in pediatric AIDS patients receiving highly active antiretroviral therapy (HAART) / 中华儿科杂志
Chinese Journal of Pediatrics
;
(12): 49-52, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-286144
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of CD38 and HLA-DR on CD8(+) T cells in pediatric AIDS patients receiving highly active antiretroviral therapy (HAART) and the relationship of immune activation and disease progression.</p><p><b>METHODS</b>A cross-section study of 194 pediatric AIDS patients receiving HAART was carried out and 52 age-matched healthy children were recruited as control. The percentage of CD4(+), CD8(+), CD8(+)/CD38(+) and CD8(+)/HLA-DR(+) T cells was tested using flow cytometry, and HIV-RNA in plasma was detected by quantitative RT-PCR.</p><p><b>RESULTS</b>One hundred and ninety-four pediatric AIDS patients were divided into two groups according to the viral load 59 patients with VL ≥ 400 copies/ml and 135 patients with VL < 400 copies/ml. The percentage of CD8(+)/CD38(+) and CD8(+)/HLA-DR(+) T cells of patients with VL ≥ 400 copies/ml was significantly higher than that of patients with VL < 400 copies/ml (P < 0.05). Of patients with VL < 400 copies/ml, the percentage of CD8(+)/CD38(+) T cells was nearly normal, and the percentage of CD8(+)/HLA-DR(+) T cells was higher than normal level (P < 0.05). There was a positive correlation between percentage of CD8(+)/CD38(+) and of CD8(+)/HLA-DR(+)T cells and viral load (R = 0.403, P = 0.03 for the former and R = 0.569, P = 0.09 for the later).</p><p><b>CONCLUSIONS</b>Effective HAART could decrease immune activation of HIV-infected children significantly. And there was a positive correlation between percentage of CD8(+)/CD38(+) and of CD8(+)/HLA-DR(+)T cells and viral load, suggesting that the two indicators might be used as the substitution of viral load in resource-limited areas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
HLA-DR Antigens
/
Case-Control Studies
/
Acquired Immunodeficiency Syndrome
/
CD8-Positive T-Lymphocytes
/
Viral Load
/
Antiretroviral Therapy, Highly Active
/
Drug Therapy
/
Allergy and Immunology
/
ADP-ribosyl Cyclase 1
Type of study:
Observational study
Limits:
Adolescent
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pediatrics
Year:
2011
Type:
Article
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