Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 686-688, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-286226
ABSTRACT
Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes. Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100,GRACE and IPL-41 with 10% serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAml in medium TC-100 with serum 1%, lactalbumin hydrolysate and yeastolate was well.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Adaptation, Physiological
/
Culture Media, Serum-Free
/
Culture Media
/
Cell Biology
/
Cell Proliferation
/
Lepidoptera
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS