A novel binary vector to get marker-free transgenic plant / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 550-554, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-286251
ABSTRACT
A novel practical binary vector to get marker-free transgenic plant was constructed. The estrogen-inducible Cre/loxP DNA recombination system was adopted in this system. All non-target genes located between two identical orientation loxP sites could be excised from the transgenic genome by the Cre expression. In order to analyze this system, the target gene, GUS expression box (CaMV35sGUS), was inserted in the MCS outside the region franked by two loxP sites. Then it was introduced into the tobaccos. Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Nicotiana
/
Molecular Sequence Data
/
Base Sequence
/
Plants, Genetically Modified
/
Integrases
/
Genetic Vectors
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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