The effect of oncolytic adenovirus on human umbilical vein endothelial cell / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 397-402, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-286277
ABSTRACT
The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Umbilical Veins
/
Cells, Cultured
/
Adenoviridae
/
Promoter Regions, Genetic
/
Adenovirus E1A Proteins
/
Cell Biology
/
Endothelial Cells
/
Cell Proliferation
/
Oncolytic Viruses
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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