Application of glycosyl fluorides in the study on glycosidases / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 351-360, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-286284
ABSTRACT
Glycosyl fluorides are becoming increasingly important molecules for the study on glycosidases. Firstly, glycosyl fluorides act as substrates for glycosidases hydrolysis. Scecondly, the installation of fluorine elsewhere on the carbohydrate ring modifies the properties of the glycosyl fluoride so that the resultant compounds act as mechanism-based inhibitors to label enzymes in the active site, allowing identification of the catalytic nucleophile. Furthermore, glycosyl fluorides also act as donors for transglycosylation by retaining glycolides. Finally, glycosyl fluorides of the wrong anomeric configuration could be used by retaining glycosidase mutants such as glycosynthases and thioglycosynthases to synthesize carbohydrate with high yields(normally 60% to approximately 90%). Fundamental and applied research in biology, glycobiology and nanobiotechnology would benefit from the possibility of synthesizing tailor-made oligo-/poly-saccharides.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Substrate Specificity
/
Chemistry
/
Glycosyltransferases
/
Enzyme Inhibitors
/
Fluorides
/
Glucosidases
/
Glycoside Hydrolases
/
Glycosides
/
Hydrolysis
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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