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Protective Effect of Total Flavones of Bidens pilosa L. on IgA1 Induced Injury of HUVECs in Henoch-Schönlein Purpura Children Patients / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 183-187, 2016.
Article in Chinese | WPRIM | ID: wpr-286313
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective effect and mechanism of total flavones of Bidens pilosa L. (TFB) on IgA1 induced injury of venous endothelial cells in Henoch-Schönlein purpura (HSP) children patients. METHODS Human umbilical venous endothelial cells (HUVECs) were taken as subject. They were intervened by normal IgA1 and HSP children patients' serum IgA1, and added with different concentrations TFB at the same time. Then they were divided into the blank control group, the normal control group, the HSP IgA1 group, and HSP IgA1 plus TFB (1.0, 0.5, 0.25 mg/mL) groups. Levels of TNF-α and IL-8 in supernate were detected by ELISA. The NO level was detected by nitrate reductase method. mRNA and protein expressions of NF-κB and ICAM-1 in HUVECs were detected by fluorescent quantitative PCR and Western blot respectively.</p><p><b>RESULTS</b>Compared with the normal control group and the blank control group, levels of IL-8, TNF-α, and NO all significantly increased in the HSP group (P < 0.05). Compared with the HSP group, levels of IL-8, TNF-α, and NO significantly decreased after intervention of TFB (1.0 and 0.5 mg/mL; P < 0.05, P < 0.01). Results of fluorescent quantitative PCR and Western blot showed, as compared with the blank control group and the normal control group, mRNA and protein expressions of NF-κB and ICAM-1 in HSP children patients' serum IgA1 induced venous endothelial cells significantly increased with statistical difference (P < 0.05, P < 0.01). Compared with the HSP group, mRNA and protein expressions of NF-KB and ICAM-1 were obviously down-regulated after intervention of TFB (1.0, 0.5, 0.25 mg/mL), with statistical difference (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>TFB could protect vascular damage by inhibiting in vivo high expression of NF-κB, reducing the production of IL-8, TNF-α, and NO in vascular endothelial cells of HSP children patients.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / IgA Vasculitis / Blood / Immunoglobulin A / RNA, Messenger / Chemistry / Interleukin-8 / NF-kappa B / Tumor Necrosis Factor-alpha / Intercellular Adhesion Molecule-1 Limits: Child / Humans Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / IgA Vasculitis / Blood / Immunoglobulin A / RNA, Messenger / Chemistry / Interleukin-8 / NF-kappa B / Tumor Necrosis Factor-alpha / Intercellular Adhesion Molecule-1 Limits: Child / Humans Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2016 Type: Article