Meiotic analysis of spermatogenic cells in severe oligoasthenoteratozoospermia with chromosome 13 rearrangement / 中华男科学杂志
National Journal of Andrology
;
(12): 793-796, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-286423
ABSTRACT
<p><b>OBJECTIVE</b>To explore the possible mechanisms of spermatogenic arrest in severe oligoasthenoteratozoospermia induced by supernumerary, ring-neocentric 13q12.3 --> 13q22 chromosome and reciprocal deletion.</p><p><b>METHODS</b>We performed a genomic-wide high-density oaCGH analysis for a case of oligoasthenoteratozoospermia with abnormal chromosome 13 to characterize the breakpoints of the chromosome involved or the gene deletion caused by the rearrangement. We also conducted a fluorescence in situ hybridization analysis on the germ cells using probes of 13q14/13qter to observe the pairing condition of homologous chromosome 13.</p><p><b>RESULTS</b>We identified by oaCGH analysis a microdeletion of 4 consecutive probes (A_16_P19757882, A_16_P02744617, A_14_ P108858 and A_16_P02744687 at chr13q12.3 27979261 --> 28039191) with 59.93 kb between the FLT1 and POMP genes, with no annotated genes in the deleted region. The signals of 13q14 and 13qter were separated from each other in 90% of all the primary spermatocytes examined, indicating the unpairing of homologous chromosome 13 or synapse failure.</p><p><b>CONCLUSION</b>Chromosomal rearrangement-induced spermatogenesis failure is caused by the unpairing of the homologous chromosomes involved in the first meiotic division of germ cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligospermia
/
Spermatogenesis
/
Chromosomes, Human, Pair 13
/
Chromosome Aberrations
/
Asthenozoospermia
/
Azoospermia
/
Comparative Genomic Hybridization
/
Chromosome Breakpoints
/
Genetics
/
Meiosis
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2012
Type:
Article
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