IFN-gamma enzyme-linked immunospot assay versus PPD tuberculin skin test in the diagnosis of tuberculous epididymitis / 中华男科学杂志
National Journal of Andrology
; (12): 534-537, 2012.
Article
in Zh
| WPRIM
| ID: wpr-286466
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the potential application of IFN-gamma enzyme-linked immunospot (ELISPOT) assay in the diagnosis of tuberculous epididymitis (TE) by comparing ELISPOT assay with the traditional purified protein derivative (PPD) tuberculin skin test.</p><p><b>METHODS</b>We examined 13 TE patients using an in-house ELISPOT kit, another 11 TE patients by PPD skin testing, and 57 healthy male volunteers by parallel test with both the methods.</p><p><b>RESULTS</b>Twelve (92.3%) of the 13 TE cases were positive on ELISPOT assay, and 10 (90.9%) of the 11 TE cases positive on PPD skin test, with no statistically significant differences between the two groups (P > 0.05). Among the 57 healthy male volunteers, 8 (14.0%) were positive on ELISPOT, and 28 (49.1%) positive on PPD test, the latter significantly higher than the former (P < 0.001).</p><p><b>CONCLUSION</b>In terms of sensitivity, ELISPOT assay is similar to PPD test in the examination of tuberculous epididymitis. As for specificity, ELISPOT assay seems better than PPD test in differentiating tuberculous epididymitis patients from healthy males.</p>
Full text:
1
Index:
WPRIM
Main subject:
Tuberculin
/
Tuberculosis, Male Genital
/
Enzyme-Linked Immunosorbent Assay
/
Tuberculin Test
/
Case-Control Studies
/
Interferon-gamma
/
Diagnosis
/
Epididymitis
Type of study:
Diagnostic_studies
/
Observational_studies
Limits:
Adult
/
Humans
/
Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2012
Type:
Article