C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 987-992, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286612
ABSTRACT
We studied the immunogenicity of pseudorabies virus gC DNA vaccination by fusing the murine complement C3d receptor binding domain. First, pseudorabies virus gC gene was linked to four copies of C3d receptor binding domain (M284), and then cloned into the vector pcDNA3.1 to construct the recombinant plasmid sgC-M284. Through the experiment of immunized BALB/c mice, we found that the enzyme linked immunosorbent assay (ELISA) antibody titer for sgC-M284 was 17-fold higher than that for sgC alone, and protective rate of mice was augmented from 25% to 88% after lethal dose PrV (316 LD50) challenge. In addition, the IL-4 levels for sgC-M284 immunization approached that for the pseudorabies virus inactivated vaccine. In conclusion, we demonstrated murine C3d receptor binding domain fusion significantly increased Th2-biased immune response by inducing IL-4 production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Swine
/
Binding Sites
/
Recombinant Proteins
/
Complement C3d
/
Viral Envelope Proteins
/
Viral Fusion Proteins
/
Adjuvants, Immunologic
/
Interleukin-4
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS