Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 946-952, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286617
ABSTRACT
Candida glycerinogenes WL2002-5 (C.g) is an important industrial strain for glycerol production. To further improve glycerol production, we reconstructed a binary vector pCAM3300-zeocin-CgGPD1, introduced it to Agrobacterium tumefaciens LBA4404 by electroporation, and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation (ATMT). After 96 h fermentation with glucose as the substrate, we screened a transformant named C.g-G8 with high glycerol production. Compared with the wild strain, the glucose consumption rate of C.g-G8 and the glycerol production were 12.97% and 18.06% higher, respectively. During the fermentation, the activity of glycerol-3-phosphate dehydrogenase of C.g-G8 was 27.55% higher than that of the wild strain. The recombinant Candida glycerinogenes with high glycerol production was successful constructed by ATMT method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Transformation, Genetic
/
Candida
/
Agrobacterium tumefaciens
/
Electroporation
/
Fermentation
/
Genetics
/
Glycerol
/
Glycerolphosphate Dehydrogenase
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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