Screening and characterization of lipase from a metagenome library of dairy rumen microflora / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 869-874, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286630
ABSTRACT
Using lipase segregation agar containing trioleoylglycerol, we obtained 18 lipase positive clones by screening from a metagenome library of dairy rumen microflora containing 15,360 clones. The average insert size of lipase positive clones was about 60 kb. Lipase enzyme activity analysis by p-NPP method indicated that Lipase6, Lipase7 and Lipase8 had higher lipolytic activities to substrates of p-nitrophenyl palmitate (C16), p-nitrophenyl alaurate (C12) and p-nitrophenyl palmitate (C16) respectively. The optimum pH of Lipase 6, Lipase 7 and Lipase 8 were 7.5. The halflife period of Lipase 8 with the value of 15 min in 70 degrees C decreased with the increase of temperature. In conclusion, the lipases screened in this study had different substrates specificity and good thermo stability, which laid a basis for large-scale industrial application.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rumen
/
Substrate Specificity
/
Temperature
/
Bacteria
/
Gene Library
/
Cloning, Molecular
/
Metagenome
/
Genetics
/
Lipase
/
Metabolism
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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