Construction of anti-H5N1 virus chimeric igA antibody gene and its expression in CHO cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 714-719, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286652
ABSTRACT
Abstract To express human-mouse chimeric IgA antibody directed against H5N1 virus, an anti-H5N1 chimeric IgA antibody gene was constructed by joining the light and heavy chain variable region genes and the corresponding signal peptide coding sequences of the anti-H5N1 mouse monoclonal antibody H5N1-HA with the coding sequences of the constant region of the human IgA2 heavy chain and Kappa chain respectively. Then the full-length chimeric light and heavy chain expressing plasmids pEF-IGHA9 and pEF-IGK9 were constructed and transfected into the CHO/dhfr cells. The chimeric IgA antibody expression was confirmed by ELISA, SDS-PAGE and Western blotting. The successful expression of this anti-H5N1 chimeric IgA may help to provide a stand for developing passive immunological agents for H5N1 virus infection prophylaxis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Immunoglobulin A
/
Cricetulus
/
CHO Cells
/
Chimerism
/
Allergy and Immunology
/
Influenza A Virus, H5N1 Subtype
/
Genetics
/
Antibodies, Monoclonal
/
Antibodies, Viral
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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