Development of single base extension-tags microarray for the detection of food-borne pathogens / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 554-559, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286674
ABSTRACT
We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and scanned for fluorescence intensity. The experiment showed a specific and simultaneous detection of eight food-borne pathogens. The system limits is 0.1 pg for a genomic DNA and 5x10(2) CFU/mL for Salmonella typhimurium cultures. The single base extension-tags assay can be used to detect food-borne pathogens rapidly and accurately with a high sensitivity, and provide an efficient way for diagnosis and control of disease caused by food-borne pathogens.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Salmonella typhimurium
/
Staphylococcus aureus
/
Vibrio parahaemolyticus
/
Food Contamination
/
Oligonucleotide Array Sequence Analysis
/
Food Microbiology
/
Listeria monocytogenes
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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