Establishment of a GFP-based cellular model for screening novel proteasome inhibitors / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 452-456, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286689
ABSTRACT
To establish a green fluorescent protein (GFP)-based cellular model for screening proteasome inhibitors from compounds including extracts from Traditional Chinese Medicinal herbs, we transfected A549 cells with lentivirus expression vector pGC-E1-ZU1-GFP, and selected clones stably expressing ZU1-GFP. The A549-ZU1-GFP cells were treated with PS-341 for 24 h, and the accumulation of GFP was analyzed by fluorescence microscope. We found that the fluorescence intensity of A549-ZU1-GFP cells treated with PS-341 was significantly increased as compared to the control. We screened for proteasome inhibitors from compounds including some from Traditional Chinese Medicinal herbs, and the data suggested a few compounds could be novel proteasome inhibitors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Protease Inhibitors
/
Pyrazines
/
Boronic Acids
/
Drugs, Chinese Herbal
/
Cell Line
/
Lentivirus
/
Green Fluorescent Proteins
/
Drug Evaluation, Preclinical
/
Bortezomib
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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