Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 428-434, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-286693
ABSTRACT
One pair of primers were designed and synthesized based on the cDNA sequence encoding Homo sapiens poly (ADP-ribose) polymerase family, member 10 (PARP10) reported on the GenBank. The cDNA sequence encoding PARP10 was cloned from 293FT cell by RT-PCR. Then the RT-PCR product was cloned into pCMV-Myc and pEGFP-C1 plasmids. The interaction between PARP10 and beta-actin was identified through immuno-precipitation and laser confocal microscopy. Extensive expression of PARP10 in mouse tissues was confirmed by RT-PCR. Besides, Western blotting analysis indicated that cell injury caused by UV treatment could promote the expression of PARP10. The results in this paper would benefit further study of PARP10.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Stress, Physiological
/
Ultraviolet Rays
/
Tissue Distribution
/
Proto-Oncogene Proteins
/
Actins
/
Poly(ADP-ribose) Polymerases
/
Protein Interaction Domains and Motifs
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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