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Role of epithelial sodium channel in rat osteoclast differentiation and bone resorption / 南方医科大学学报
Journal of Southern Medical University ; (12): 1148-1152, 2016.
Article in Chinese | WPRIM | ID: wpr-286831
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of epithelial sodium channel (ENaC) in regulating the functional activity of osteoclasts.</p><p><b>METHODS</b>Multinucleated osteoclasts were obtained by inducing the differentiation of rat bone marrow cells with macrophage colony-stimulating factor (M-CSF) and RANKL. The osteoclasts were exposed to different concentrations of the ENaC inhibitor amiloride, and the expression of ENaC on osteoclasts was examined using immunofluorescence technique. The osteoclasts were identified with tartrate-resistant acid phosphatase (TRAP) staining, and the positive cells were incubated with fresh bovine femoral bone slices and the number of bone absorption pits was counted by computer-aided image processing. RT-PCR was performed to analyze the expression of cathepsin K in the osteoclasts.</p><p><b>RESULTS</b>s Exposure to different concentrations of amiloride significantly inhibited the expression of ENaC and reduced the number of TRAP-positive osteoclasts. Exposure of the osteoclasts to amiloride also reduced the number of bone resorption pits on bone slices and the expression of osteoclast-specific gene cathepsin K.</p><p><b>CONCLUSION</b>s ENaC may participate in the regulation of osteoclast differentiation and bone resorption, suggesting its role in functional regulation of the osteoclasts and a possibly new signaling pathway related with ENaC regulation for modulating bone metabolism.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Bone Resorption / Bone Marrow Cells / Signal Transduction / Cell Differentiation / Macrophage Colony-Stimulating Factor / Cell Biology / RANK Ligand / Epithelial Sodium Channels / Cathepsin K Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Bone Resorption / Bone Marrow Cells / Signal Transduction / Cell Differentiation / Macrophage Colony-Stimulating Factor / Cell Biology / RANK Ligand / Epithelial Sodium Channels / Cathepsin K Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2016 Type: Article