Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity / 南方医科大学学报
Journal of Southern Medical University
;
(12): 814-818, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-286893
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism.</p><p><b>METHODS</b>MCF-7 cells treated with 100, 200, and 400 µmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS.</p><p><b>RESULTS</b>DADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells.</p><p><b>CONCLUSION</b>DADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Vimentin
/
Breast Neoplasms
/
Cadherins
/
Gene Expression Regulation, Neoplastic
/
Matrix Metalloproteinase 9
/
MAP Kinase Signaling System
/
Mitogen-Activated Protein Kinase 11
/
Disulfides
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article
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