Effect of spleen tyrosine kinase on the proliferation of pulmonary vascular smooth muscle cells in rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 886-890, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-286957
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of spleen tyrosine kinase (Syk) in rat pulmonary vascular smooth muscle cells (PVSMCs) proliferation induced by platelet-derived growth factor-BB (PDGF-BB).</p><p><b>METHODS</b>PVSMCs from male Sprague-Dawley rats were cultured in vitro and the cells of passages 3-5 were used in the experiment. PVSMCs were stimulated by PDGF-BB and were treated with three different doses of piceatannol, a Syk selective inhibitor. Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) assay. DNA synthesis was measured by ³H-thymidine incorporation (³H-TdR). Cellular cycle was observed by flow cytometry. Syk mRNA and protein expression were detected using real-time quantitative PCR and Western blot, respectively.</p><p><b>RESULTS</b>The expression of Syk protein of PVSMCs was significantly up-regulated following PDGF-BB stimulation. PDGF-BB stimulation dramatically increased PVSMCs proliferation. After piceatannol treatment, both Syk mRNA and protein expression decreased and the proliferation of PVSMCs was inhibited in a dose-dependent manner.</p><p><b>CONCLUSIONS</b>Syk may promote PVSMCs proliferation induced by PDGF-BB.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Pulmonary Artery
/
Stilbenes
/
Protein-Tyrosine Kinases
/
Platelet-Derived Growth Factor
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Proto-Oncogene Proteins c-sis
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS