Effect of antisense oligonucleotides of ryanodine receptor on proliferation and Ca2+i of airway smooth muscle cells / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To investigate the effect of antisense oligonucleotides (ASON) of ryanodine receptor on proliferation and [Ca2+]i concentration of airway smooth muscle cells (ASMCs).</p><p><b>METHODS</b>ASMCs were cultivated with collagen enzyme digestion method. Different concentrations of ASON were added to the cultures with Lipofectamine 2000 to observe the ASMCs proliferation using MTS/PES method. The changes of ASMCs [Ca2+]i were also observed by flow cytometry. The expression of mRNA of subtypes of RyR was assayed by RT-PCR.</p><p><b>RESULTS</b>RyR ASON restrained the proliferation of ASMCs, decreased the expression of RyR and reduced the concentration of [Ca2+]i.</p><p><b>CONCLUSION</b>RyR ASON could inhibit the proliferation of ASMCs by influencing the concentration of [Ca2+]i after excited.</p>