Effects of acute hypoxia and intermittent hypoxic acclimatization on vascular endothelial growth factor gene expression in HepG2 cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 274-277, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-287036
ABSTRACT
<p><b>AIM</b>To explore the influence of acute hypoxia and intermittent hypoxic acclimatization on vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1alpha (HIF-1alpha) gene expression in HepG2 cells underlying their possible biological significance.</p><p><b>METHODS</b>HepG2 was cultured in 1% O2 for 24 hours, then in 21% O2 for another 24 hours, which composed a hypoxic exposure cycle. After 6 cycles, HepG2 cells reached the status of hypoxic acclimatization. Gene transcription and translation of VEGF and HIF-1alpha were detected with Northern blot and Western blot methods.</p><p><b>RESULTS</b>Acute hypoxia could induce gene transcription and translation of VEGF and HIF-1alpha. After intermittent hypoxia acclimatization, the contents of VEGF and HIF-1alpha mRNA were 108.6% +/- 17.7% and 116.7% +/- 19.8% of those in normoxic control cells, while the protein contents were significantly increased to 1.4 and 2.7 times of those in control cells, respectively (P < 0.05). The protein expression levels of VEGF and HIF-1alpha were decreased in cells subjected to hypoxia acclimatization compared to cells treated with acute hypoxia.</p><p><b>CONCLUSION</b>When HepG2 cells reached the status of hypoxic acclimatization, the acute hypoxia-induced increment of VEGF gene transcription and translation in cells were inhibited, in which HIF-1alpha might play an important role.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Nuclear Proteins
/
Cell Hypoxia
/
Gene Expression
/
Vascular Endothelial Growth Factor A
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Hep G2 Cells
/
Genetics
/
Acclimatization
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2005
Type:
Article
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