Proteomics analysis on stressed myocardium injury-related proteins / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 171-174, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-287066
ABSTRACT
<p><b>AIM</b>To probe the related proteins to stress-induced myocardium injury.</p><p><b>METHODS</b>After establishment of a myocardium injury model induced by restraint stress in rats, myocardium proteins of restraint stress-treated and untreated rats were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) and SDS-PAGE two-dimensional electrophoresis respectively. The alterative protein spots were analyzed by Image Master 3.01 software and identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.</p><p><b>RESULTS</b>Proteomics analysis showed that there were 10 proteins were significantly influenced by restraint stress in rat myocardium. After stress, proteins, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, similar to dihydrolipoamide S-succinyltransferase, mitochondrial aldehyde dehydrogenase, H (+)-transporting ATP synthase, albumin, myosin heavy chain and apolipoprotein A-I precursor showed increased expression. Mitochondrial aconitase and uncoupling protein UCP-3 showed decreased expression.</p><p><b>CONCLUSION</b>These differential expressive proteins might be involved in stress-induced injury to myocardium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stress, Physiological
/
Restraint, Physical
/
Rats, Wistar
/
Proteomics
/
Disease Models, Animal
/
Heat-Shock Proteins
/
Metabolism
/
Myocardium
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2005
Type:
Article
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