Whole-cell recording of potassium and sodium currents in cortical, hippocampal, and sympathetic ganglia neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 105-110, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-287086
ABSTRACT
<p><b>AIM</b>To establish the culturing of cortical, hippocampal and sympathetic ganglia neurons derived from neonatal rats and the whole-cell recording techniques of potassium and sodium currents in cultured neurons.</p><p><b>METHODS</b>Cortex, hippocampus and sympathetic ganglion were isolated from neonatal rats (1-3 days) and digested by trypsin (0.125%). The neurons were cultured in polylysine covered petri dish (O35 mm) with DEME medium(high glucose) for seven days for electrophysiological experiments.</p><p><b>RESULTS</b>The neurons cultured by this method were in good conditions and could be sealed easily in whole-cell recording. I(Na), I(A), and I(K) could be recorded respectively in cultured neurons by different recording paradigms in Ca2+ -free extracellular solution.</p><p><b>CONCLUSION</b>It is a practicable and economical method for electrophysiological study of nervous system.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Calcium Channels
/
Potassium Channels
/
Sodium Channels
/
Cells, Cultured
/
Cerebral Cortex
/
Rats, Wistar
/
Patch-Clamp Techniques
/
Cell Biology
/
Ganglia, Sympathetic
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2005
Type:
Article
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