The effects of antisenes oligodeoxynucleotide on the cyclic nucleotide monophosphates in smooth muscle cells of human corpus cavernosum / 中华男科学杂志
National Journal of Andrology
;
(12): 88-91, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-287239
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effects of antisense oligodeoxynucleotide(ASON) on the cyclic nucleotide monophosphates (cNMP) in smooth muscle cells of human corpus cavernosum, and provide experimental groundwork for the gene therapy of erectile dysfunction.</p><p><b>METHODS</b>PDE5 gene ASON(containing exon 1) was transfected into the corpus cavernosum smooth muscle cells with the presence of liposome DOTAP. Another sense oligodeoxynucleotide(SON) and 1% of bovine serum were also transducted into the cells as controls. Two of cNMP, cAMP and cGMP, were probed and measured by ELISA at 1, 2, 4, 6, 10, 24 and 48 h after transfection.</p><p><b>RESULTS</b>After transfection, the level of cGMP(1-6 h) in human corpus cavernosum smooth muscle cells was significantly higher than that in controls(P < 0.01).</p><p><b>CONCLUSIONS</b>The PDE5 gene ASON had been showed to manifest stimulative effect on the cGMP in smooth muscle cells of human corpus cavernosum in vitro, and it provides experimental groundwork for the gene therapy of erectile dysfunction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penis
/
Pharmacology
/
Cyclic AMP
/
3',5'-Cyclic-GMP Phosphodiesterases
/
Cyclic GMP
/
Oligodeoxyribonucleotides, Antisense
/
Cell Biology
/
Cyclic Nucleotide Phosphodiesterases, Type 5
/
Genetics
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2002
Type:
Article
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