Oridonin induced U937 cell apoptosis through ERK pathway / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1856-1859, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-287270
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanisms of oridonin-induced U937 cell apoptosis, and to examine the role of ERK MAPK.</p><p><b>METHOD</b>MTT, Hoechst 33258 staining, DNA agarose gel electrophoresis and Western blot analysis were used.</p><p><b>RESULT</b>Oridonin inhibited U937 cell growth in a time- and dose-dependent manner. Apoptotic bodies were found with Hoechst 33258 staining after treatment with 27 micromol x L(-1) oridonin. Simultaneously, ERK phosphorylation was significant. ERK inhibitor PD98059 partially blocked the growth-inhibitory effect as well as DNA fragmentation. The expression of antiapoptotic mitochondrial protein Bcl-XL decreased time-dependently, and that of proapoptotic protein Bax increased. However, PD98059 reversed the effect of oridonin on Bcl-XL and Bax.</p><p><b>CONCLUSION</b>Oridonin induces U937 cell apoptosis through activation of ERK and alteration of the ratio of Bax/Bcl-XL.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphorylation
/
Plants, Medicinal
/
Flavonoids
/
Chemistry
/
Apoptosis
/
U937 Cells
/
Isodon
/
Diterpenes, Kaurane
/
Extracellular Signal-Regulated MAP Kinases
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article
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