Role of mitochondria in Tubeimoside I-mediated apoptosis in human cervical carcinoma HeLa cell line / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line.</p><p><b>METHOD</b>Mitochondrial transmembrane potentia (deltapsim) was assayed by flow cytometry. Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA. Cytochrome c (Cyt c) was detected by Western blotting.</p><p><b>RESULT</b>The results showed that TBMS1 induced apoptosis in HeLa cells, that TBMS1 decreased deltapsim and facilitated Cyt c release, and that TBMS1 induced apoptosis of HeLa cells dose-and time-dependently in accordance with increase of cytosolic Cyt c. TBMS1 had direct effect on isolated rat mitochondria which induced a time- and dose-dependent release of Cyt c from mitochondria. The results also showed that CsA protected partly HeLa cells from the effect of TBMS1.</p><p><b>CONCLUSION</b>The mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma HeLa cell line apoptosis.</p>