Effect of osteopontin silencing by lentivirus-mediated delivery of siRNA on glioma cell invasion and apoptosis / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 525-528, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-287383
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells.</p><p><b>METHODS</b>The invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively.</p><p><b>RESULTS</b>Osteopontin small interference RNA (siRNA) inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated.</p><p><b>CONCLUSION</b>Osteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl 2 level. It suggests that osteopontin plays an important role in human glioma progression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
Gene Transfer Techniques
/
Lentivirus
/
Gene Silencing
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Osteopontin
/
Genetic Vectors
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2009
Type:
Article
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