Sperm-fluorescence in situ hybridization analysis in patients with pericentric inversions of Y chromosome / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 54-56, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-287455
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the sex chromosome meiotic segregation in inv(Y) patients by fluorescence in situ hybridization (FISH).</p><p><b>METHODS</b>Conventional cytogenetic procedures (GTG and CBG banding) and FISH were performed on metaphase chromosome. Three-color FISH was performed on sperm samples using a probe mixture containing CEPX, Tel Xp/Yp and Tel Xq/Yq to investigate the sex chromosome segregation of five inv(Y) (p11.1q11.2) carriers. A healthy man with normal semen parameters was used as control.</p><p><b>RESULTS</b>There was no statistical difference in the abnormal sex chromosome number and recombination frequencies in each spermatozoon from the patient in comparison with that in the control.</p><p><b>CONCLUSION</b>There was no apparent sex chromosome abnormality in the sperm of the inv(Y) (p11.1q11.2) carriers. Sperm-FISH allows further understanding of the sex chromosome segregation pattern and an accurate genetic counseling.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Recombination, Genetic
/
Sex Chromosome Aberrations
/
Spermatozoa
/
Case-Control Studies
/
In Situ Hybridization, Fluorescence
/
Chromosomes, Human, Y
/
Genetics
/
Chromosome Inversion
/
Meiosis
Type of study:
Observational study
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2009
Type:
Article
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