Roles of bFGF in endothelial cell tube formation induced by xuefu zhuyu decoction under non-anoxia condition / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the roles of basic fibroblast growth factor (bFGF) on tube formation induced by xuefu zhuyu decoction (XZD) under non-anoxia condition.</p><p><b>METHODS</b>Using serum pharmacology technique, endothelial cell line ECV304 cells were incubated in routine 95% O2. ECV304 cells were intervened by 1.25%, 2.50%, and 5.00% XZD containing serums and the vehicle serum for 48 h. The effects of XZD on tube formation, bFGF contents and its transcription levels were assessed by in vitro tube formation assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcriptase polymerase chain reaction (RT-PCR), respectively.</p><p><b>RESULTS</b>Three concentrations of XZD containing serums could not only obviously promote the tube formation bFGF level, but also up-regulate bFGF contents in the supernate and its transcription levels. The shapes of lumens were more regular in those induced by 1.25% and 2.50% XZD containing serums.</p><p><b>CONCLUSION</b>XZD induced angiogenesis via up-regulating the bFGF expression under non-anoxia condition.</p>