Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 48-51, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-287821
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate anti-HEV diagnostic kits by experimental infecting rhesus monkeys with HEV.</p><p><b>METHODS</b>Eight rhesus monkeys were infected with genotype 1 and 4 HEV separately. The alanine aminotransferase (ALT) level of all monkeys were detected before and after the process of infection. HEV RNA in stool specimens was tested by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Anti-HEV IgG in serum was detected by GL-IgG and WT-IgG.</p><p><b>RESULTS</b>HEV RNA presented in the stool of all the 8 monkeys after infection. The ALT level of 1 monkey infected with genotype 1 HEV and 2 monkeys infected with genotype 4 HEV appeared abnormally after infection. Tested by GL-IgG, 2 of the 4 monkeys infected with genotype 1 HEV and 1 of 4 monkeys infected with genotype 4 HEV seroconverted to anti-HEV IgG. However, when tested by WT-IgG, all the infected monkeys seroconverted to anti-HEV IgG. The anti-HEV IgG tested by WT-IgG was positive during the whole observation period,and the anti-HEV IgG measured by GL-IgG only remained 12 weeks after infection. Detected by GL-IgG and WT-IgG, seropositive conversion of the anti-HEV IgG happened almost at the same time.</p><p><b>CONCLUSION</b>Both GL-IgG and WT-IgG could detect the anti-HEV IgG of experimentally infected rhesus monkeys but the WT-IgG had a higher sensitivity for detection of anti-HEV IgG than</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Blood
/
Immunoglobulin G
/
Hepatitis E virus
/
Hepatitis E
/
Reverse Transcriptase Polymerase Chain Reaction
/
Alanine Transaminase
/
Disease Models, Animal
/
Allergy and Immunology
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2008
Type:
Article
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