Study on conditions of seed germination of Cistanche / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of fluridone concentration, stimulating period, temperature and salt on the seed germination of three species of Cistanche.</p><p><b>METHOD</b>The seeds were cultured in Petri dish, and the germination percentage was counted.</p><p><b>RESULT</b>The highest germination percentage was observed in Cistanche tubulosa, C. deserticola, C. sala seeds pre-treated by 0.1 mg x L(-1) fluridone for 24-29 h. The optimal temperature for the seeds germination of three species of Cistanche was at 20-30 degrees C, and the seeds did not germinate at sub-or supraoptimal temperatures (5 and 35 degrees C). The salt tolerance of C. sala seeds was strong, and the critical value of NaCl concentration was 0.04 mol x L(-1). By contrast, C. tubulosa and C. deserticola seeds were more sensitive to the salt stress, the critical value of NaCl concentration was 0.02 mol x L(-1).</p><p><b>CONCLUSION</b>The optimal germination condition and the method of testing germination percentage of three species of Cistanche seeds are as follow: the seeds are pre-treated by 0.1 mg x L(-1) fluridone for 24 h and then cultured at 20-30 degrees C in salt solution which concentration is lower than 0.02 mol x L(-1).</p>