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Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-287879
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.</p><p><b>METHOD</b>The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75250.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).</p><p><b>RESULT</b>Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).</p><p><b>CONCLUSION</b>The method could be used for quality control of Bile Arisaema.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Reference Standards / Scattering, Radiation / Bile / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Arisaema / Plant Tubers Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Reference Standards / Scattering, Radiation / Bile / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Arisaema / Plant Tubers Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2007 Type: Article