Determination of citrinin in red kojic by HPCE / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.</p><p><b>METHOD</b>Red kojic was extracted with the mixtrue of Toluene and ethyl acetate (7030). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.</p><p><b>RESULT</b>Regression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.</p><p><b>CONCLUSION</b>The method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.</p>