Drug resistance of colon cancer cells to 5-fluorouracil mediated by microRNA-21 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 620-624, 2015.
Article
in Zh
| WPRIM
| ID: wpr-288022
Responsible library:
WPRO
ABSTRACT
OBJECTIVE To explore downstream regulatory pathway of microRNA-21 (miR-21) in colon cancer cells (RKO) through detecting miR-21 and its target PDCD4, and the influence of miR-21 regulation on the sensitivity of RKO cells to 5-fluorouracil (5-FU). METHODS 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the effect of 5-FU on the viability of RKO cells with knockout of miR-21 or high expression of PDCD4. Real-time was used to determine the expression of PDCD4, ABCC5 and CD44 in RKO cell after knockout of miR-21. RESULTS MTT assay reveals that the IC50 of 5-FU in RKO-WT cells (52.82 ± 0.06 umol/L) was about 67% higher than in miR-21 knockout cells (32.23 ± 0.05 umol/L) (P < 0.05), and the apoptosis ratio elevated after knockout of miR-21. High expression of PDCD4, a target gene of miR-21, can negatively regulate the expression of ABC transporter ABCC5 and the stem cell marker CD44. CONCLUSION MiR-21 can mediate the drug resistance to 5-FU by inhibiting its target PDCD4, which can regulate the expression of ABCC5 and CD44 genes.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Physiology
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RNA-Binding Proteins
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Colonic Neoplasms
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ATP-Binding Cassette Transporters
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Drug Resistance, Neoplasm
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Hyaluronan Receptors
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MicroRNAs
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Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2015
Type:
Article