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ALK fusion gene assessment by fully automatic immunohistochemistry in non-small cell lung cancer / 中华病理学杂志
Chinese Journal of Pathology ; (12): 95-98, 2014.
Article in Chinese | WPRIM | ID: wpr-288158
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the sensitivity and specificity of fully automated immunohistochemistry (IHC), with comparison to FISH, in the detection of EML4-ALK rearrangement in lung adenocarcinoma (ADC); and the use of IHC as a pre-screening tool.</p><p><b>METHODS</b>A total of 404 paraffin-embedded NSCLC samples from surgical resections were tested by IHC with Ventana anti-ALK rabbit monoclonal antibody (D5F3) and ultrasensitive detection kit. ALK rearrangement was further confirmed by FISH.</p><p><b>RESULTS</b>Twenty-nine of 404 lung ADCs (7.2%) were positive for ALK by IHC. ALK positive tumor cells demonstrated strong and diffused granular cytoplasmic staining. All the ALK IHC-positive cases were confirmed to harbor ALK rearrangement by FISH. None of the ALK IHC-negative cases was FISH-positive.</p><p><b>CONCLUSIONS</b>IHC can effectively detect ALK rearrangement in lung cancer. It may provide a reliable and cost-effective diagnostic approach in routine pathologic laboratories for the identification of suitable candidates for ALK-targeted therapy.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Immunohistochemistry / Gene Rearrangement / Adenocarcinoma / Oncogene Proteins, Fusion / Sensitivity and Specificity / In Situ Hybridization, Fluorescence / Carcinoma, Non-Small-Cell Lung / Genetics / Lung Neoplasms / Metabolism Type of study: Diagnostic study Limits: Adult / Aged / Aged80 / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Pathology Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Immunohistochemistry / Gene Rearrangement / Adenocarcinoma / Oncogene Proteins, Fusion / Sensitivity and Specificity / In Situ Hybridization, Fluorescence / Carcinoma, Non-Small-Cell Lung / Genetics / Lung Neoplasms / Metabolism Type of study: Diagnostic study Limits: Adult / Aged / Aged80 / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Pathology Year: 2014 Type: Article