Computer construction and analysis of protein models of the mutant gammaD-crystallin gene / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 738-741, 2005.
Article
in English
| WPRIM
| ID: wpr-288343
ABSTRACT
<p><b>BACKGROUND</b>Gammad-crystallin plays an important role in human cataract formation. Being highly stable, gammaD-crystallin proteins are composed of two domains. In this study we constructed and analyzed protein models of the mutant gammaD-crystallin gene, which caused a special fasciculiform congenital cataract affecting a large Chinese family.</p><p><b>METHODS</b>gammaD-crystallin protein structure was predicted by Swiss-Model software using bovine gammaD-crystallin as a template and Prospect software using human betab2-crystallin as a template. The models were observed with a Swiss-Pdb viewer.</p><p><b>RESULTS</b>The mutant gammaD-crystallin structure predicted by the Swiss-Model software showed that proline23 was an exposed surface residue and P23T change made a decreased hydrogen bond distance between threonine23 and asparagine49. The mutant gammaD-crystallin structure predicted by the Prospect software showed that the P23T change exerted a significant effect on the protein's tertiary structure and yielded hydrogen bonds with aspartic acid21, asparagine24, asparagine49 and serine74.</p><p><b>CONCLUSION</b>The mutant gammaD-crystallin gene has a significant effect on the protein's tertiary structure, supporting that alteration of gamma-crystallin plays an important role in human cataract formation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Computer Simulation
/
Models, Molecular
/
Chemistry
/
Protein Structure, Tertiary
/
Gamma-Crystallins
/
Genetics
/
Hydrogen Bonding
/
Mutation
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2005
Type:
Article
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