Establishment of rat integrated discrete multiple organ cell culture (IdMOC) model / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 482-487, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-288378
ABSTRACT
<p><b>OBJECTIVE</b>To establish the integrated discrete multiple organ cell culture (IdMOC) system.</p><p><b>METHODS</b>Rat primary cell of hepatocyte, nephrocyte, cardiomyocytes, alveolar macrophage, dermal fibroblasts were isolated by collagenase digestion, separation of bronchial lavage, two-step digestion method and cultured respectively, with monolayer culture. To establish the integrated discrete multiple organ cell culture (IdMOC) system, glass slides of five different cells were used to the same dish with 10% FBS DMEM medium cultured 7d, using MTT comparison primary cells cultured alone and cocultured when growth.</p><p><b>RESULTS</b>Established rat hepatocytes, renal cell, cardiomyocyte, alveolar macrophages, dermal fibroblasts separation method was stable, cell separation survival rate was about 90.0%. Hepatocytes separation survival rate 90.3% ,renal cell separation survival rate 91.9%, cardiomyocyte separation survival rate 93.0% and beating rate indifference curve among 3d-15d, alveolar macrophages cell separation survival rate 90.8%, dermal fibroblasts cell separation survival rate 92.7%. Five primary cells multiple organ cells coculture showed cocultured cell growth proliferation well, cultured alone and cocultured cells growth curve basic coincide.</p><p><b>CONCLUSION</b>Established rat multiple organ cell co-culture is successful.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Macrophages, Alveolar
/
Rats, Sprague-Dawley
/
Cell Culture Techniques
/
Hepatocytes
/
Cell Biology
/
Myocytes, Cardiac
/
Epithelial Cells
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2010
Type:
Article
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