Rat kidney mitochondrial damage induced by acute cadmium administration / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 345-349, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-288435
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate effect of acute cadmium administration on mitochondria from rat kidney.</p><p><b>METHODS</b>24 male Sprague-Dawley (SD) rats were randomly divided into four groups. Four groups of rats were injected with saline, 0.6, 1.2, and 1.8 mg/kg weight subcutaneously, once daily, for 5 days, respectively. Ultrastructural change of rat kidney mitochondria was observed, and respiration function, membrane potential, mitochondria swelling, and superoxide level were determined.</p><p><b>RESULTS</b>Ultrastructural changes included matrix vacuolation, swelling and condensation of mitochondria. In group of 1.8 mg/kg body weight, the oxygen consumption rate during state 3 respiration [(6.25 +/- 0.61) nmol/L O2 x min(-1) x mg(-1)] and RCR value (2.45 +/- 0.23) were significantly lower than those of control group [(9.66 +/- 1.16) nmol/L O2 x min(-1) x mg(-1)] (P < 0.05), indicating respiration inhibition. The membrane potential and superoxide level of the same group were 85.89% +/- 3.82% and 116.33% +/- 3.06% of control values (P < 0.05), respectively.</p><p><b>CONCLUSIONS</b>Acute cadmium administration can cause rat kidney mitochondrial damage in a dose-effect manner, including inhibition of respiration, dissipation of membrane potential, swelling of mitochondria matrix. Such damage might be related to the increase of mitochondrial free radical.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Pathology
/
Cadmium
/
Reactive Oxygen Species
/
Rats, Sprague-Dawley
/
Toxicity Tests, Acute
/
Toxicity
/
Membrane Potential, Mitochondrial
/
Kidney
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS