Determination of multiple constituents in Wuling capsules by HPLC simultaneously / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 218-221, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-288669
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the content determination of 5-methylmellein, 5-hydroxymellein, 5-carboxylmellein and genistein in Wuling capsules simultaneously by HPLC.</p><p><b>METHOD</b>Four components were determined by HPLC on a Kromasil KR100-5C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile and 0. 2% phosphoric acid as mobile phase in a gradient elution. The flow rate was 1.0 mL x m min(-1), and the column temperature was set at 30 degrees C.</p><p><b>RESULT</b>5-hydroxymellein showed a good linear relationship at the range of 7.86-157.2 ng, the average recovery was 101.0% with RSD 1.3%. 5-carboxylmellein showed a good linear relationship at the range of 9.57-191.4 ng, the average recovery was 98.6% with RSD 1.5%. Genistein showed a good linear relationship at the range of 28.80-576.0 ng, the average recovery was 98.6% with RSD 1.8%. 5-methylmellein showed a good linear relationship at the range of 21.46-429.2 ng, the average recovery was 99.2% with RSD 1.8%.</p><p><b>CONCLUSION</b>The established method is feasible and the repeatability is good. The method can be used for quality control of Wuling capsules.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Capsules
/
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Genistein
/
Methods
/
Ochratoxins
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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