Transcriptional regulation of dentin sialophosphoprotein by c-Jun/c-Fos / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 67-69, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-289002
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.</p><p><b>METHODS</b>Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.</p><p><b>RESULTS</b>c-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter.</p><p><b>CONCLUSION</b>These findings suggest c-Jun and c-Fos down-regulated the transcription of DSPP gene as a transcriptional factor in odontoblast.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphoproteins
/
Sialoglycoproteins
/
Transfection
/
Cell Line
/
Gene Expression Regulation
/
Extracellular Matrix Proteins
/
Promoter Regions, Genetic
/
Odontoblasts
Limits:
Animals
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2006
Type:
Article
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