Expression of epidermal growth factor receptor in human periodontal ligament cells during their mineralization in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 11-14, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-289019
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of epidermal growth factor receptor (EGFR) during the mineralization of human periodontal ligament cells (hPDLC) in vitro.</p><p><b>METHODS</b>Studies using specific antibodies to immunolocalize EGFR in the mineral differentiating hPDLC were undertaken to investigate the different expression during the inducing process. In situ hybridization and RT-PCR technique were used to investigate the transcripts encoding the protein of EGFR.</p><p><b>RESULTS</b>The results showed that immunocytochemical labeling gradually decreased following the elong of the induce time, downing to nearly negative at the 4th week and the signal of EGFR transcripts was weaker in the induced hPDLC than that in uninduced.</p><p><b>CONCLUSION</b>EGFR has a negative regulation function during the mineralization of hPDLC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Ligament
/
In Vitro Techniques
/
Cells, Cultured
/
In Situ Hybridization
/
ErbB Receptors
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS