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Construction of the recombinant adenovirus vector expressing human CD40 ligand gene / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 534-536, 2005.
Article in Chinese | WPRIM | ID: wpr-289022
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector expressing hCD40L gene and explore it in the use of anti-tumor gene therapy.</p><p><b>METHODS</b>1,900 bp gene fragment was obtained form plasmid pORF-hCD40L by Xho I/Swa I cutting and then cloned directionally into the pShuttle plasmid, finally, the resultant plasmid was digested by restriction endonnuclease PmeI and subsequently cotransformtion into BJ5183 cells with the adenoviral backbone pAdEasy-1 to obtain the homologous recombinant and then the recombinant was packaged in the 293 cells. Some methods such as PCR and endonulease digestion were employed to identify the recombinant adenovirus.</p><p><b>RESULTS</b>The evidences of endonulease digestion and PCR analysis confirmed that recombinant hCD40L gene was correctly inserted into adenovirus vector.</p><p><b>CONCLUSION</b>The adenoviral vector which expressed hCD40L gene was constructed. It provides an experimental basis for studies on it expression in the mammalian cells and in tumor gene therapy.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Genetic Therapy / Adenoviridae / Adenoviruses, Human / Polymerase Chain Reaction / CD40 Ligand / Genetic Vectors Type of study: Prognostic study Limits: Animals / Humans Language: Chinese Journal: West China Journal of Stomatology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Genetic Therapy / Adenoviridae / Adenoviruses, Human / Polymerase Chain Reaction / CD40 Ligand / Genetic Vectors Type of study: Prognostic study Limits: Animals / Humans Language: Chinese Journal: West China Journal of Stomatology Year: 2005 Type: Article