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Potassium channel blocker regulates the proliferation of prostatic epithelial cells in SD rats / 中华男科学杂志
National Journal of Andrology ; (12): 138-142, 2007.
Article in Chinese | WPRIM | ID: wpr-289032
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effect of potassium channel blocker (tetraethylammonium [TEA], aminopyridine [4-AP], glibenclamide [Glib]) on the proliferation of SD rat prostatic epithelial cells in vitro.</p><p><b>METHODS</b>The primary culture was prepared by collagenase dissociation of minced prostatic tissues. Cells were cultured in serum-free prostate epithelial cell growth media and identified by immunocytochemical studies. TEA and 4-AP at the concentration of 1, 5 and 10 mmol/L and Glib at the concentration of 10, 50 and 100 mol/L were added, and after 24, 48 and 72 hours of culturing, a cell column diagram was drawn and the cell number counted. The post-passage cell growth was observed by MTT assay and Hoechst33258 nucleus staining.</p><p><b>RESULTS</b>The cultured cells showed the typical morphological features of epithelia, with positive stain. MTT assay and Hoechst33258 staining showed that TEA, 4-AP and Glib at the increasing concentration effected different degrees of proliferation of prostatic epithelial cells after 24, 48 and 72 h (P < 0.01).</p><p><b>CONCLUSION</b>The potassium channel blocker is a direct physiological regulator of the proliferation of SD rat prostatic epithelial cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Prostate / Cells, Cultured / Rats, Sprague-Dawley / Cell Biology / Potassium Channel Blockers / Cell Proliferation / Epithelial Cells Limits: Animals Language: Chinese Journal: National Journal of Andrology Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Prostate / Cells, Cultured / Rats, Sprague-Dawley / Cell Biology / Potassium Channel Blockers / Cell Proliferation / Epithelial Cells Limits: Animals Language: Chinese Journal: National Journal of Andrology Year: 2007 Type: Article