Inhibition activity of semenogelin and its peptides to human spermatozoa / 中华男科学杂志
National Journal of Andrology
;
(12): 42-45, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-289049
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibition activity of Semenogelin (Sg) and its different peptides to human spermatozoa.</p><p><b>METHODS</b>Human Sg DNA and its N-terminal Sg and C-terminal Sg DNA were cloned into PET-100 vector. Positive colonies were screened and transformed into E. Coli BL21 (DE3). Recombinant Sg and its peptides were induced and expressed in high competent E. coli BL21 (DE3) , and purified by 50% Ni-NTA column. Inhibition activity assay was done by adding 4 different concentrations of semenogelin and its two peptides, 0, 1, 5 and 10 ng/microl, to human spermatozoa.</p><p><b>RESULTS</b>The peptide of Semenogelin that inhibits the activity of human spermatozoa was located in its N-terminal fragment. C-terminal Sg did not inhibit the activity of spermatozoa.</p><p><b>CONCLUSION</b>N-terminal Sg is the inhibition peptide of the whole molecular Sg. During semen liquefaction, this peptide should be cut off from the surface of human spermatozoa before they move forward.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sperm Motility
/
Recombinant Proteins
/
Cloning, Molecular
/
Seminal Vesicle Secretory Proteins
/
Dose-Response Relationship, Drug
/
Escherichia coli
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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